%0 Journal Article
%T Construction of the glucose isomerase deficient strain of Streptomyces M1033 by homologous recombination
%A XU Chong
%A LIAO Jun
%A XIE Wen
%A CHENG Yang
%A ZHU Guoping
%A YANG Yonghui
%A CHEN Chenglu
%A TAO Limei
%A NIU Liwen
%A WANG Yuzhen
%A
%J 科学通报(英文版)
%D 2000
%I
%X After the establishment of the transformation conditions ofStreptomyces diastaticus No.7 Strain M1033, the integration plasmid pXW for homologous recombination, which contains a 600 bp fragment of incompleteGI (G138P. G247D) gene, has been constructed in order to realize the stable overexpression of theGI (G138P. G247D) which is valuable for large-scale industrial production. The Gigene’s disruption has been realized by pXW’s integration into M1033 chromosomes via homologous recombination andGI deficient strain ofStreptomyces M1033 has been obtained. The reliability of introduction of mutation has been proved by analysis of recombinant fragment and affirmance of existence of the mutation, as well as detection of the stability of the deficient strain.
%K glucose isomerase
%K Streptomyces
%K homologous recombination
%K gene disruption
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=F4A99B8C353432AE&yid=9806D0D4EAA9BED3&vid=94E7F66E6C42FA23&iid=CA4FD0336C81A37A&sid=0401E2DB1F51F8DE&eid=09ABD5535D9B6D45&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=13