%0 Journal Article
%T A novel method of DNA shuffling without PCR process
%A WANG Qiang
%A LIU Qiuyun
%A LI Gang & LI Baojian Key Laboratory of Gene Engineering of Ministry of Education
%A School of Life Sciences
%A Zhongshan University
%A Guangzhou
%A China Correspondence should be addressed to Li Baojian
%A <br>WANGQiang
%A LIUQiuyun
%A LIGang
%A LIBaojian
%J 科学通报(英文版)
%D 2004
%I 
%X Most DNA shuffling methods currently used require PCR process. A novel method of DNA shuffling without PCR process is described, taking advantage of the feature of some restriction enzymes whose recognition sites differ from their cleavage sites, thus giving rise to different cohesive ends.These cohesive ends can be rejoined at their native sites from different parental sequences, generating new sequences with various combinations of mutations.
%K DNA shuffling
%K restriction enzyme
%K tissue plasminogen activator<br>DNA滑移
%K 限制酶
%K 组织纤溶酶原
%K 并行序列
%K 试管内进化
%K 识别位
%K 变异
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=40CEB5E169DA3FC1EE5E8FDE02A52210&yid=D0E58B75BFD8E51C&vid=2A3781E88AB1776F&iid=DF92D298D3FF1E6E&sid=128B7AEF80A42C95&eid=09F7C8D609E885AE&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=13