%0 Journal Article
%T Neuropeptide Y receptors in a protozoa Stylonychia mytilus<br>
%A Xiaoyang He
%A Xiaoyun Zhang
%A Yunhong Zhen
%A <br>
%J 科学通报(英文版)
%D 1998
%I 
%X Radio ligand binding assays(RLBA) were used to study neuropeptide Y (NPY) receptors in a protozoaStylonychia mytilus. The experimental results showed that 2–3 × 103/mLStylonychia cells incubated in Pringsheim solution which contained3H-NPY could specifically bind3H-NPY and concoraitantly present saturable characteristic. This suggested thatStylonychia possessed some specific binding sites for NPY. Scatchard transformations of binding assay for the NPY receptors at 25°C are compatible with the specific activity of 42. 47 fmol/103 cells and the binding equilibrium constant of 0. 113 nmol/L. The data of125I-NPY binding assay to the membrane protein extract ofStylonychia indicated that there was a significant difference between the amount of total bound and nonspecific bound of125I-NPY. This result indicated that NPY receptors were probably localized mainly on the cell membrane.
%K Stylonychia
%K NPY receptor
%K radio ligand binding assay(RLBA)<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=583EEFA40390231E5152E0166CDDE230&yid=8CAA3A429E3EA654&vid=BE33CC7147FEFCA4&iid=B31275AF3241DB2D&sid=3F419E61BD389CC8&eid=3F419E61BD389CC8&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=8