%0 Journal Article
%T Detection of Salmonella by Surface Plasmon Resonance
%A Benjamin Barlen
%A Saikat Datta Mazumdar
%A Olga Lezrich
%A Peter K£¿mpfer
%A Michael Keusgen
%J Sensors
%D 2007
%I MDPI AG
%R 10.3390/s7081427
%X This study explores the possibility of simultaneous and specific detection ofSalmonella serovars by surface plasmon resonance (SPR). The Plasmonic£¿ SPR device wasused to develop this rapid assay. The sandwich immunoassay involves the use of apolyclonal anti-Salmonella antibody to simultaneous capture multiple Salmonella serovarspresent in a sample. This is followed by specific detection of the captured serovars usingO-specific anti-Salmonella antibodies. Milk spiked with Salmonella Typhimurium andSalmonella Enteritidis was used as a model system to establish the assay. The assay wasfurther extended to sequentially differentiate between the two Salmonella serovars on asingle SPR chip in a single channel. The assay was proved to work without any additionaldilution or clean-up steps. The sample volume requirement for the assay is only 10 ¦ÌL. Thelower limits of detection for Salmonella Typhimurium and Salmonella Enteritidis were2.50¡Á105 cells mL-1 and 2.50¡Á108 cells mL-1, respectively.
%K Salmonella serovars
%K lipopolysaccharide (LPS)
%K O-specific antibody
%K sequential detection
%K Plasmonic£¿ surface plasmon resonance (SPR) devic
%U http://www.mdpi.com/1424-8220/7/8/1427