%0 Journal Article
%T Secretory expression of the fusion protein composed of human IL-2 and PreS antigen of hepatitis B virus in mammalian cells<br>
%A Zhangguo Chen
%A Dalong Ma
%A Jing Wang
%A Yingmei Zhang
%A Yong Yuan
%A Wenling Han
%A Hongtao Liu
%A <br>
%J 科学通报(英文版)
%D 1998
%I 
%X In order to make entire HBV pmSAg secrete from mammalian cells, we conatmcted an eukaryotic expression vector by using leader sequence of human interleukin-2 (IL-2) as secretory signal peptide, and using high hydmphilic amino acids as the linker between IL-2 C end and preSAg N end. As a result, the IL-2preS fusing protein could be secreted from mamalian cells transfected with the reconstructed vector and the expression efficiency was identical to that of natural IL-2. It was considered that the retentive effect of preSlAg could be successfully bypassed. The results not only laid a theoretical and practical foundation for constructing specific gene vaccine against HBV persistent infection, but also supplied experimental evidence for studying modulation of protein secretory expression.
%K hepatitis B
%K gene vaccine
%K gene immunization
%K preS antigen
%K mammalian cells
%K secretion<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=0578EBB7C7E994A51AA3CDE052C6B10F&yid=8CAA3A429E3EA654&vid=BE33CC7147FEFCA4&iid=9CF7A0430CBB2DFD&sid=DBEE434FCBFED297&eid=DBEE434FCBFED297&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=6