%0 Journal Article
%T Antibody Immobilization on Waveguides Using aFlow¨CThrough System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM
%A Viswaprakash Nanduri
%A Giyoung Kim
%A Mark T. Morgan
%A Daniel Ess
%A Byoung-Kwon Hahm
%A Aparna Kothapalli
%A Angela Valadez
%A Tao Geng
%A Arun K. Bhunia
%J Sensors
%D 2006
%I MDPI AG
%R 10.3390/s6080808
%X Recent outbreaks of food borne illnesses continue to support the need for rapidand sensitive methods for detection of foodborne pathogens. A method for detecting Listeriamonocytogenes in food samples was developed using an automated fiber-optic-basedimmunosensor, RAPTORTM. Detection of L. monocytogenes in phosphate buffered saline(PBS) was performed to evaluate both static and flow through antibody immobilizationmethods for capture antibodies in a sandwich assay. Subsequent detection in frankfurtersamples was conducted using a flow through immobilization system. A two stage blockingusing biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed toreduce the non-specific binding. The sandwich assay using static or flow through mode ofantibody immobilization could detect 1
%K Biosensor
%K Listeria monocytogenes
%K fiber optic sensor
%K immunosensor
%K RAPTOR
%U http://www.mdpi.com/1424-8220/6/8/808