%0 Journal Article
%T Immobilization of Mucor miehei Lipase onto Macroporous Aminated Polyethersulfone Membrane for Enzymatic Reactions
%A Nurrahmi Handayani
%A Katja Loos
%A Deana Wahyuningrum
%A Buchari
%A Muhammad Ali Zulfikar
%J Membranes
%D 2012
%I MDPI AG
%R 10.3390/membranes2020198
%X Immobilization of enzymes is one of the most promising methods in enzyme performance enhancement, including stability, recovery, and reusability. However, investigation of suitable solid support in enzyme immobilization is still a scientific challenge. Polyethersulfone (PES) and aminated PES (PES每NH 2) were successfully synthesized as novel materials for immobilization. Membranes with various pore sizes (from 10每600 nm) based on synthesized PES and PES每NH 2 polymers were successfully fabricated to be applied as bioreactors to increase the immobilized lipase performances. The influence of pore sizes, concentration of additives, and the functional groups that are attached on the PES backbone on enzyme loading and enzyme activity was studied. The largest enzyme loading was obtained by Mucor miehei lipase immobilized onto a PES每NH 2 membrane composed of 10% of PES每NH 2, 8% of dibutyl phthalate (DBP), and 5% of polyethylene glycol (PEG) (872.62 米g/cm 2). Hydrolytic activity of the immobilized lipases indicated that the activities of biocatalysts are not significantly decreased by immobilization. From the reusability test, the lipase immobilized onto PES每NH 2 showed a better constancy than the lipase immobilized onto PES (the percent recovery of the activity of the lipases immobilized onto PES每NH 2 and PES are 97.16% and 95.37%, respectively), which indicates that this novel material has the potential to be developed as a bioreactor for enzymatic reactions.
%K aminated PES
%K solid support
%K Mucor miehei
%K enzymatic reactions
%K lipase immobilization
%U http://www.mdpi.com/2077-0375/2/2/198