%0 Journal Article
%T Physicochemical Characterization and Cytotoxic Activity Evaluation of Hydroxymethylferrocene:¦Â-Cyclodextrin Inclusion Complex
%A Rosa Iacovino
%A Jolanda Valentina Caso
%A Filomena Rapuano
%A Agostino Russo
%A Marina Isidori
%A Margherita Lavorgna
%A Gaetano Malgieri
%A Carla Isernia
%J Molecules
%D 2012
%I MDPI AG
%R 10.3390/molecules17056056
%X An inclusion complex of hydroxymethylferrocene (FeMeOH) with ¦Â-cyclodextrin (¦Â-CD) was prepared in the solid state by different techniques such as physical mixture, coprecipitation, kneading and freeze-drying. The formation of the inclusion complex was confirmed by X-ray Powder Diffractometry and Fourier Transform-Infrared spectroscopy. In aqueous solution, the 1:1 stoichiometry was established by a Job plot. The inclusion complex formation was also investigated by NMR and the stability constant (Kb) of the complex was determined to be 478 M£¿1, which is in agreement with that obtained with UV-Vis tritation (Kb = 541.3 M£¿1). The phase solubility study showed a diagram classified as BS type and that the solubility of FeMeOH was slightly increased in the presence of ¦Â-CD. Furthermore, utilizing phase solubility diagram data, the Kb was estimated to be equal to 528.0 M£¿1. The cytotoxic activity of FeMeOH and its complexation product with ¦Â-CD was determined using the MTT-assay on MDA-MB-231 cell line, showing that the inclusion complex has a higher capability of inhibiting cell growth compared to that of pure FeMeOH.
%K ¦Â-cyclodextrin
%K hydroxymethylferrocene
%K inclusion complex
%K stability constant
%K phase solubility diagram
%K cytotoxicity
%U http://www.mdpi.com/1420-3049/17/5/6056