%0 Journal Article %T Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 %A Ricardo Lievano %A Herminia In¨¦s P¨¦rez %A Norberto Manjarrez %A Aida Sol¨ªs %A Myrna Sol¨ªs-Oba %J Molecules %D 2012 %I MDPI AG %R 10.3390/molecules17033148 %X A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but the reactions are not enantioselective and low ee values were obtained. However, (R)-ibuprofen was isolated with >99% ee by a deracemisation process catalysed by N. corallina B-276. This is the first report of this kind of catalysis with this microorganism. %K Nocardia corallina %K deracemisation %K ibuprofen %K nitrile hydratase %K amidase %U http://www.mdpi.com/1420-3049/17/3/3148