%0 Journal Article
%T The protoplast preparation and regeneration of Trametes hirsuta and laccase production of monokaryons<br>毛栓菌原生质体制备和再生及单核菌株产漆酶特性
%A LI Jiang
%A YUAN Yue-Xiang
%A YAN Zhi-Ying
%A LIU Xiao-Feng
%A LIAO Yin-Zhang
%A LI Na
%A <br>李江
%A 袁月祥
%A 闫志英
%A 刘晓风
%A 廖银章
%A 李娜
%J 菌物学报
%D 2012
%I 
%X Trametes hirsuta is very effective on degradation of lignin, and is proved to be of high value in biofuel, pulping, feed and other industries. To get the monokaryons of T. hirsuta YJ-9-1, we investigated the effect of several factors on the protoplast preparation and regeneration, such as growth medium type, fungal age, hydrolysis time, centrifugal speed for purification of protoplasts and regeneration medium type. The monokaryons were identified by using DAPI staining and clamp connection deficiency method, and ligninolytic enzyme production characteristics of the monokaryons were investigated. The protoplast forming number and regeneration rate reached 5.0×106/mL and 9.1% respectively under the optimal conditions, viz. yeast glucose malt agar (YGM) medium for fungus growth, fungal age of 88h, hydrolisis duration of 1h, centrifugal speed of 4,000r/min, and yeast glucose malt sucrose agar (YGMS) medium for protoplasts regeneration. Three strains of monokaryons were obtained and the strain named D-2-1 had a higher laccase production level than that of YJ-9-1, and the laccase activity of D-2-1 reached 771.67U/L on day 12, which is 1.5 times as high as that of YJ-9-1.
%K white-rot fungi
%K Trametes hirsuta
%K optimal conditions of protoplast preparation
%K laccase activity<br>白腐菌，毛栓菌，原生质体制备优化条件，漆酶活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=3B2CD3BB807ED8FAD9F3E04650EEA35B&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=331211A5F5616413&eid=91C9056D8E8856E0&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=34