%0 Journal Article
%T Fast molecular detection of the pathogen of sunflower black stem<br>向日葵黑茎病菌的快速分子检测
%A SONG N
%A CHEN Wei-Min
%A YANG Jia-Rong
%A HU Xiao-Ping
%A <br>宋娜
%A 陈卫民
%A 杨家荣
%A 胡小平
%J 菌物学报
%D 2012
%I 
%X The diseased stems, leaves and faceplates of sunflower with black stem symptom were sampled in Xinjiang Autonomous Region, China. Twenty fungal isolates were finally isolated from the materials. Isolates XJ011 and XJ111 were found to be pathogenic to sunflower plants. ITS sequence analysis indicated the isolates XJ011 and XJ111 were Phoma macdonaldii. A pair of specific primers 320FOR/320REV were designed for rDNA-ITS of P. macdonaldii according to polymorphism region, and it can be used to detect P. macdonaldii specifically with highly sensitivity of 1fg concentration.
%K pathogenicity
%K specificity primers
%K rDNA-ITS region
%K sunflower<br>致病性
%K 特异性引物
%K rDNA-ITS区
%K 向日葵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=7CA476D0C6D2F7274675D0EE50171F01&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=039DCCB9394D9766&eid=50B6AC44200581A5&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0