%0 Journal Article
%T RAPD analysis of cucurbit powdery mildew fungus in Heilongjiang Province<br>黑龙江省葫芦科白粉病菌RAPD分析
%A WEI Zun-Miao
%A GAO Peng
%A WANG Xue-Zheng
%A LUAN Fei-Shi
%A ZHANG Xiao-Bo
%A <br>魏尊苗
%A 高鹏
%A 王学征
%A 栾非时
%A 张晓博
%J 菌物学报
%D 2011
%I 
%X 17 samples of powdery mildew were collected and separated from diseased melon, cucumber, pumpkin and watermelon in greenhouses or open field in various ecological regions of Heilongjiang Province in 2010. They were investigated for physiological races by using standard differential hosts of powdery mildew. Based on their resistant or susceptible types on 13 differential hosts, three races on Cucurbitaceae were preliminarily confirmed, including races 1, race N1 and a new one of Podosphaera xanthii in Heilongjiang Province. Race 1 was dominant in Heilongjiang. Thirteen isolates of powdery mildew were evaluated by using random amplified polymorphic DNA (RAPD) with 10 primers selected from 119 random primers. A total of 157 bands were amplified, including 138 polymorphic bands with 97.89% polymorphic band frequency. The RAPD amplifying results show that high genetic diversity existed among the isolates of P. xanthii on Cucurbitaceae in Heilongjiang. After analysis by software NTSYS-PC, the similarity coefficient of the 13 isolates varied from 0.52 to 0.57. The UPGM dendrogram divided the 13 isolates into 4 groups at the similarity coefficient of 0.60. The isolates of race 1 were partly clustered into the same group, and the new race was clustered into the same group with partial race 1 isolates; two isolates of race N1 were not clustered into the same group with partial race 1 isolates. The isolates originated from the same geographic regions or hosts were also not clustered into the same group. It is indicated that no obvious association was found between DNA polymorphism and pathogen races in biological tests on cucurbits. Meanwhile, no association was found between the results of RAPD analysis and any other parameter analysis such as geographic regions, greenhouse types or host origins.
%K Podosphaera xanthii
%K physiological race identification
%K NTSYS-PC clustering analysis
%K genetic diversity<br>单囊壳白粉菌，生理小种鉴定，NTSYS-PC分析，遗传多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=3EA1D73CA3DE3CAABC4BFBC776FFB745&yid=9377ED8094509821&vid=340AC2BF8E7AB4FD&iid=E158A972A605785F&sid=D5970ECA7D10A7B1&eid=0A8675156EB60B87&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=44