%0 Journal Article
%T Phenotype analysis of the T-DNA insertion mutant Focr4-1562 of Fusarium oxysporum f. sp. cubense race 4 and its gene-knockout mutant<br>尖镰孢古巴专化型4号小种T-DNA插入突变体Focr4-1562及其基因敲除子的生物学表型研究
%A WU Fei-Hong
%A ZENG Tao
%A CHEN Han-Qing
%A ZENG Hui-Cai
%A PENG Ming
%A <br>吴飞宏
%A 曾涛
%A 陈汉清
%A 曾会才
%A 彭明
%J 菌物学报
%D 2012
%I 
%X Fusarium oxysporum f.sp.cubense race 4(Focr4) is considered as one of the most destructive pathogens of the world distributed soil-borne banana.Its pathogenesis has not been clearly understood.The pathogenicity of mutant Focr4-1562 was significantly weakened after T-DNA insertion in its gene sequence.The inserted exogenous gene caused changes in functions of some genes in the △Focr4-1562.By means of cellophane penetration test as well as pathogenic test by inoculating live leaves and roots,changes in pathogenic phenotype of the mutant Focr4-1562 and △Focr4-1562 were compared with the wild type Focr4.The mutant Focr4-1562 and △Focr4-1562 could not grow through cellophane,and didn’t cause any disease spots and brown coloration in corm vascular bundle.The results showed that the optimum growth temperature of the wild type Focr4,Focr4-1562 and △Focr4-1562 were all at 28℃,and their optimum growth pH was 7.0 to 8.0.No evident difference was found in their spore morphology,but the sporulation of △Focr4-1562 was obviously delayed,and its sporulation capacity was significantly decreased.The Focr4-1562 and △Focr4-1562 were more resistant to cell wall degrading enzyme than the wild type Focr4-193-6.It is concluded that the knockout of the pathogenic gene is responsible for conidial production and cell wall formation of the wild type Focr4-193-6.
%K Banana wilt disease
%K T-DNA insertion mutant
%K gene knockout
%K biological characters<br>香蕉枯萎病
%K T-DNA
%K 插入突变体
%K 基因敲除
%K 生物学特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=7CA476D0C6D2F727E7A83A1A5F827371&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=E158A972A605785F&sid=E04FC1B5BC47587B&eid=106103EB0EA31435&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0