%0 Journal Article
%T Cloning and expression analysis of a polyketide synthase gene vv-alb from the edible straw mushroom, Volvariella volvacea<br>草菇聚酮合酶编码基因vv-alb的克隆及其表达的初步分析
%A LIN Nan
%A MAO Wen-Jun
%A WANG Hong
%A FENG Ai-Ping
%A BAO Da-Peng
%A <br>林楠
%A 茅文俊
%A 汪虹
%A 冯爱萍
%A 鲍大鹏
%J 菌物学报
%D 2012
%I 
%X Polyketides are secondary metabolites that exhibit a broad range of biological activities. The key enzyme involved in biosynthesis is polyketide synthase (PKS). We report the cloning of a putative PKS gene (vv-alb) by nested PCR using degenerate primers and chromosome walking from the edible straw mushroom, Volvariella volvacea. The gene vv-alb is 6,704bp in size and contains a 6,279bp coding region (encoding 2,093 amino acid residues) and eight introns. The encoded product of the vv-alb gene contains four functional domains: ketosynthase (KS), acyl transferase (AT), thioesterase (TE) and acyl carrier protein (ACP), the typical characteristics of PKS. Real-time quantitative PCR analysis revealed that vv-alb expression was 5-10-fold higher in the stipe region as compared with the vegetative mycelium, primordia and pileus.
%K Volvariella volvacea
%K polyketide synthase
%K real-time quantitative PCR<br>草菇，聚酮合酶，荧光实时定量RT-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=3B2CD3BB807ED8FABCC8C0C32A8CFC82&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=E22B6B8FE86DD8F9&eid=EB552E4CFC85690B&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=16