%0 Journal Article
%T Differential proteomics analysis of degeneration on the substrate- decomposing of Agaricus bisporus<br>双孢蘑菇基质降解能力退化的差异蛋白质组学分析
%A CHEN Mei-Yuan
%A WANG Ze-Sheng
%A LIAO Jian-Hu
%A LI Hong-Rong
%A CAI Zhi-Xin
%A XIE Bao-Gui
%A <br>陈美元
%A 王泽生
%A 廖剑华
%A 李洪荣
%A 蔡志欣
%A 谢宝贵
%J 菌物学报
%D 2011
%I 
%X The technique of two-dimensional electrophoresis (2-DE or 2D-PAGE) was used to analyze the protein expression differences between the wild type Agaricus bisporus CGMCC No. 0214 and its substrate-decomposing ability degenerated strains 0214-3, 0214-5. As a result, two proteins were found to be up- and down-expressed, respectively, with obvious and repeatable profiles in both degenerated strains when grown in a liquid culture. Based on the results of MALDI-TOF/MS analysis and database searching, these two proteins were identified as an actin and a putative NADH dehydrogenase iron-sulfur protein 3, respectively. The results of this study provided a marker on the proteomics level for further study of the molecular mechanism of substrate-decomposing ability degeneration in A. bisporus.
%K edible fungus
%K proteome
%K two-dimensional electrophoresis
%K differential protein
%K MALDI-TOF/MS analysis<br>食用菌，蛋白质组，双向电泳，差异蛋白质，质谱分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=026AD26F43119EEB9745D1EADB6797B2&yid=9377ED8094509821&vid=340AC2BF8E7AB4FD&iid=38B194292C032A66&sid=37F781FD8E744761&eid=F9A6B6F259CE5121&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=19