%0 Journal Article
%T The biological synthesis, purification and characterization of an acid phytase from Aspergillus niger A-828<br>黑曲霉变异株A-828酸性植酸酶的生物合成和性质的研究
%A ZENG Ji-Jiao
%A LI Ying-Lun
%A WANG Hong-Bin
%A SHI Feng
%A YUE Li-Li
%A LI Yun-Min
%A LIU Zhi-Gang
%A JIAO Qing-Hua
%A <br>曾继蛟 李英伦 王鸿宾 石峰 岳丽丽
%A 李运敏 刘志刚 矫庆华
%J 菌物学报
%D 2009
%I 
%X Screening out a novel Aspergillus niger strain (A-828) which expresses high active acid phytase through combination mutation by Co-60 and UV. To research its enzymatic characterization, the acid phytase was purified by the steps of ultrafiltration and Bio-gel-P-150 chromatography. The purification ratio and yield was 11.2 and 29%. Result shows that its molecular weight was estimated to be about 66kDa by SDS-PAGE. The phytase activity of A-828 reached to the 66,000U/mL, 17 fold than origin. Its optimum pH of...
%K PhyB<br>纯化
%K 原牛质体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=328F2E33931FEBBF3C07B085C1A10A12&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=38B194292C032A66&sid=B7ACE2F11789CAE0&eid=E57FE519484CFB70&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=18