%0 Journal Article
%T Molecular genetic structure of the B mating-type locus of Lentinula edodes II. Molecular organization of the B mating type locus of a L. edodes monokaryon<br>香菇B交配型位点的分子遗传学结构研究II.一个香菇单核体的B位点结构的分子解析
%A CHEN Ming-Jie
%A SONG Wen-Hu
%A SONG Chun-Yan
%A ZHANG Mei-Yan
%A CHEN Xiang
%A LIN Nan
%A BAO Da-Peng
%A <br>陈明杰
%A 宋文华
%A 宋春艳
%A 张美彦
%A 陈祥
%A 林楠
%A 鲍大鹏
%J 菌物学报
%D 2011
%I 
%X In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the whole genome sequencing of the protoplast monokaryon of Lentinula edodes strain 135, to amplify STE3-like pheromone receptor gene and its flanking conserved genes in the protoplast monokaryon strain SUP2 derived from Lentinula edodes strain Suxiang and 33,655bp DNA sequence was obtained. By BlastX search, seven putative genes were identified, and three of them are pheromone receptor encoded genes. Furthermore, near to two pheromone receptor genes, four genes encoding proteins with conserved motifs of pheromone precursors were found. This study firstly reveals the molecular organization of the B mating type locus of Lentinula edodes.
%K Lentinula edodes
%K B mating type locus
%K pheromone receptor
%K pheromone precursor<br>香菇，B交配型基因，信息素受体，信息素前体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=A2252B48D295481D067FA6B86AB8BB59&yid=9377ED8094509821&vid=340AC2BF8E7AB4FD&iid=CA4FD0336C81A37A&sid=318E4CC20AED4940&eid=6AC2A205FBB0EF23&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=30