%0 Journal Article
%T Construction of rDNA-mediated stable expression vectors in Saccharomyces cerevisiae<br>rDNA介导的酿酒酵母稳定表达载体的构建
%A ZHANG Gui-Min
%A ZENG Yu-Fang
%A CHEN Ya-Lan
%A TU Jun
%A HE Jia-Heng
%A MA Li-Xin
%A <br>张桂敏
%A 曾毓芳
%A 陈雅兰
%A 屠俊
%A 何家亨
%A 马立新
%J 菌物学报
%D 2011
%I 
%X A fragment containing ori of ColE1 and bla gene was inserted in the EcoR I or Hpa I site of the rRNA gene segment from Saccharomyces cerevisiae, respectively. Based on the expression vector pYES2 and the above two recombinant fragments, two novel rDNA-mediated stable expression vectors, designated as pHBM367E/H, were constructed. The glucoamylase gene from Aspergillus niger was inserted into the vector pHBM367H and the resultant plasmid was named pHBM166. For bio-safety consideration, the plasmid pHBM166 was linearized by Hpa I digestion to remove ColE1 ori and bla gene. Digested product was transformed into S. cerevisiae Y33. Transformants showed different expression levels of glucoamylase. Stability research showed that the selected transformant can express glucoamylase stably in S. cerevisiae for at least 80 generations.
%K Saccharomyces cerevisiae
%K rDNA-mediation
%K glucoamylase
%K bio-safety
%K different expression level<br>酿酒酵母，rDNA介导，糖化酶，生物安全性，剂量效应
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=D82E30B08E6FA9B0A221DCD6087B20D3&yid=9377ED8094509821&vid=340AC2BF8E7AB4FD&iid=CA4FD0336C81A37A&sid=7C3A4C1EE6A45749&eid=94E7F66E6C42FA23&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=32