%0 Journal Article
%T Microsatellite sequences for identification of Agaricus blazei mutants induced by Co60 irradiation<br>巴氏蘑菇微卫星序列的分离及其对Co60辐射诱变菌株的鉴别
%A CHEN Jian
%A WENG Bo-Qi
%A CHEN Bin
%A LEI Jin-Gui
%A ZHU Bing-Yao
%A WANG Yi-Xiang
%A YANG Zhi-Min
%A JIANG Zhi-He
%A <br>陈坚
%A 翁伯琦
%A 陈彬
%A 雷锦桂
%A 朱炳耀
%A 王义祥
%A 杨志敏
%A 江枝和
%J 菌物学报
%D 2012
%I 
%X Based on the specifical combination between biotin and streptavidin coated magnetic beads, biotinylated probes composed of di-nucleotide repeat were used to isolate microsatellite sequences from genome of Agaricus blazei. DNA digested fragments of Agaricus blazei ligated with artificial adapters in both ends were hybridized with the labeled probes absorbed in magnetic beads. After eluting the unhybridized DNA, the enriched fragments in beads were released to establish the microsatellite library. Of 522 clones detected by PCR with corresponding repeat primers, 48 clones have shown to be positive reaction. After sequencing, 32 clones were proved to contain microsatellite sequences (SSR), with a microsatellite enrichment efficiency of 67% as against positive clones and of 6% as against the total detected clones. By deleting the duplicated and unavailable sequences, 12 microsatellite loci were selected to design primers for identifing 85 candidate mutants of Agaricus blazei induced by Co60 irradiation. The total 17 mutants could be determined by 4 pairs of primer. It demonstrates that some microsatellite loci can be applied to screen and distinguish the mutants of Agaricus blazei induced by Co60 irradiation.
%K simple sequence repeat (SSR)
%K streptavidin coated magnetic beads
%K biotinylated probes
%K basidiomycota
%K molecular marker<br>简单序列重复
%K 链霉素磁珠
%K 生物素标记探针
%K 担子菌门
%K 分子标记
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=2350B5576F89D07229E391C5CA337283&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=94C357A881DFC066&sid=80BBC722D530DB8D&eid=6452E1221020E61F&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0