%0 Journal Article
%T Optimization of low-cost medium constituents for submerged fermentation of Ganoderma lucidum by using response surface methodology<br>响应曲面法优化灵芝廉价型深层发酵培养基的研究
%A LIU Gao-Qiang
%A WANG Xiao-Ling
%A HAN Wen-Jun
%A WANG Wei
%A <br>刘高强
%A 王晓玲
%A 韩文军
%A 王卫
%J 菌物学报
%D 2009
%I 
%X To obtain a low-cost medium for submerged fermentation of Ganoderma lucidum in a large scale, four medium constituents were optimized by using central composite rotatable design (CCRD) and response surface methodology (RSM). The four factors were corn flour (x_1), wheat bran flour (x_2), soybean flour (x3) and sucrose (x_4). The results show that the mathematical model for biomass production and polysaccharide production are Y_1 = 15.1 - 0.31x_1 - 0.34x_2 + 0.36x_3 - 0.44x_4 - 1.26x_1~2 - 1.98x_2_2 - 0.85x_3~2 - 1.15x_4~2 - 0.59x_2x_3, and Y_2 = 2,0 - 0.08x_1 - 0.08x_2 + 0.04x_3 - 0.09x_4 - 1.13x_1~2 - 0.33x_2~2 - 0.08x_3~2 - 0.16x_4~2 - 0.16x_2x_3 - 0.10x_1x_4, respectively. Based on the models, the optimum medium for biomass production is corn flour 19.7g/L, wheat bran flour 11.3g/L, soybean flour 6.3g/L, and sucrose 19.5g/L; while the optimum medium for polysaccharide production is corn flour 19.6g/L, wheat bran flour 11.0g/L, soybean flour 6.7g/L, and sucrose 19.1g/L. The maximum biomass and polysaccharide in a 150-L stirred-tank bioreactor reach 16.92g/L and 1.86g/L under the optimized medium, respectively. The use of the medium can reduce the costs for both biomass and polysaccharide production by G. lucidum at large scale.
%K medicinal fungus
%K central composite rotatable design
%K polysaccharide
%K fermentation optimization
%K biomass<br>药用真菌，中心组合旋转设计法，多糖，发酵优化，生物量
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=E6E2673676B714411C814DC1B0241C5A&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=B31275AF3241DB2D&sid=95780E43ADDDE2AA&eid=6D80B994DAB4686B&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=16