%0 Journal Article
%T Effect of Disaccharide on Cryopreservation of Quality Parameters in Boar Sperm<br>双糖对冻融后猪精子质量的影响
%A HAN Ming-ming
%A JIN Yi
%A FANG Nanzhu
%A PIAO Hai-xian
%A CAO Li-peng
%A <br>韩明铭
%A 金一
%A 方南洙
%A 朴海仙
%A 曹丽朋
%J 吉林农业大学学报
%D 2010
%I 
%X In order to improve boar sperm quality parameters in frozen-thawed process,0.1 mol/L lactose,sucrose and trehalose were respectively added into the basic extenders Tris-Citric acid-Glucose(TCG).The effects of disaccharide on quality of cryopreservation of boar sperm were investigated.We found out that TCG extender with 0.1 mol/L trehalose and sucrose significantly enhanced motility,acrosome integrity rate,hypotonic expansion rate, VAP, LIN, ALH and BCF of boar sperm after freezing and thawing(P<0.05),they were 42.14% and 40.56%,51.48% and 49.84%,46.20% and 43.01%,32.8 μm/s and 33.2 μm/s,60.80% and 59.00%,1.70 μm and 1.62 μm,7.68 Hz and 7.62 Hz.The IVF rate of frozen-thawed sperm in 0.1 mol/L trehalose and sucrose is significantly higher than that of others(P<0.05). They were 41% and 38%.
%K boar sperm
%K disaccharide
%K cryopreservation
%K quality parameter<br>猪精子
%K 双糖
%K 冻融
%K 质量参数
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=74C612B26EF38BF0D4C997E3F9E167A1&aid=0D6F6AE83F5EC9175F199B6F9B214CCF&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=CA4FD0336C81A37A&sid=7AA74D31F1FF2DCE&eid=7E8E8B150580E4AB&journal_id=1000-5684&journal_name=吉林农业大学学报&referenced_num=0&reference_num=9