%0 Journal Article
%T Molecular detection of Tilletia bromi on brome grasses<br>雀麦上雀麦腥黑粉菌Tilletia bromi的分子检测
%A LIAO Fang
%A ZHAO Lei
%A LUO Jia-Feng
%A HUANG Guo-Ming
%A LIU Yue-Ting
%A LI Ming-Gang
%A <br>廖芳
%A 赵磊
%A 罗加凤
%A 黄国明
%A 刘跃庭
%A 李明刚
%J 菌物学报
%D 2009
%I 
%X Brome grasses are economically and ecologically important forage grasses, and have been frequently introduced into China these years. The smut fungi Tilletia spp. are important pathogens on these grasses and have greatly endangered grass production. There are four Tilletia species, i.e. T. controversa (TCK), T. bromi, T. bolayi and T. caries (TCT), infecting these grasses. It is difficult to separate them from each other based on the characteristics of their teliospores. In this study, the morphology, autofluorescence and germination behavior of teliospores were determined and four species, T. bromi, TCK, TCT and T. fusca with similar characteristics were selected for further analysis. Based on the nucleotide difference of a beta-tubulin tub2 gene, a molecular marker that is sequence characterized amplified region (SCAR) was developed specifically for PCR detection of T. bromi by using the DNA samples from either the mycelia or the teliospores isolated from brome grasses. The method developed in this study can be quickly and reliably employed in quarantine analysis.
%K sequence characterized amplified region (SCAR) marker
%K nested-PCR
%K molecular detection<br>SCAR分子标记，套式PCR，分子检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=20AB4A1132E90BCDC2B47468FECC83A3&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=B31275AF3241DB2D&sid=FE17D37BFC90FA6B&eid=B75DC817262E20A8&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=20