%0 Journal Article
%T GFP as a vital marker for investigation of pathogenic development of Beauveria bassiana<br>利用GFP表达系统检测球孢白僵菌侵染昆虫过程
%A JIN Kai
%A ZHANG Yong-Jun
%A LUO Zhi-Bing
%A LIN Jian-Wen
%A PEI Yan
%A <br>金凯
%A 张永军
%A 罗志兵 林健文
%A 裴炎
%J 菌物学报
%D 2008
%I 
%X The green fluorescent protein (GFP) has been expressed in a variety of organisms and has been used extensively as a marker in the study of host-pathogen interaction. We have expressed GFP in the entomopathogenic fungus Beauveria bassiana through transformation with a vector that confers resistance to phosphinothricin. Conidia expressed GFP and were readily detected by fluorescence microscopy. Fluorescent hyphae and conidia were easily distinguished in the tissue of the cabbage worm. GFP-tagged strains of B. bassiana could be used to study the developmental fate of the fungus within its insect hosts. Thus, this method is useful for screening and monitoring the engineered strains even after infection.
%K entomopathogenic fungus
%K transformation
%K frozen section
%K fluorescence detection<br>昆虫病原真菌
%K 转化
%K 冰冻切片
%K 荧光检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=D3EE4B4790E635EEF173055DF6224D8E&yid=67289AFF6305E306&vid=DB817633AA4F79B9&iid=38B194292C032A66&sid=389106FB36CA8332&eid=05340B75C67FF664&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=26