%0 Journal Article
%T CONSTRUCTION OF THE THERMOTOLERANT YEASTS BY PROTOPLAST FUSION BETWEEN KLUYVEROMYCES AND SACCHAROMYCES<br>克鲁维酵母与酿酒酵母属问原生质体融合构建高温酵母菌株*
%A WEN Tieqiao
%A <br>文铁桥
%A 赵学慧
%J 菌物学报
%D 1999
%I 
%X 通过PEG诱导碘乙酸灭活呼吸缺陷克鲁维酵母（Kluyveromycessp．Y034）原生质体与酿酒酵母（SaccharomycescerevisiaeA001）原生质体融合，获得45℃发酵产酒率高达8．7％的高温酵母菌株AY006。线粒体缺失和氯霉素抑制可显著降低高密度原生质体回复抑制效应。对融合子菌落形态、同工酶性质和高温发酵等方面分析，融合株表达了耐温和高产酒率双亲优良性状，证实其杂种特征。
%K Saccharomyces
%K Kluyveromyces
%K protoplast fusion
%K thermotolerant yeast<br>酿酒酵母
%K 克鲁维酵母
%K 原生质体融合
%K 高温酵母菌株
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=C3124CC3B60EC7EEAE7D52B85C14D412&yid=B914830F5B1D1078&vid=13553B2D12F347E8&iid=CA4FD0336C81A37A&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0