%0 Journal Article
%T Screening and Identification of Bacillus subtilis SH7 Strain against Ralstonia solanacearum<br>抗烟草青枯病菌的枯草芽孢杆菌SH7的筛选与鉴定
%A DUAN Yan-ping
%A YANG Jin-guang
%A YANG Ji-hong
%A CAO Na
%A LI Xiao
%A ZHOU Xian-sheng
%A LI Yi-qiang
%A <br>段燕平
%A 杨金广
%A 杨继洪
%A 曹娜
%A 李晓
%A 周显升
%A 李义强
%J 吉林农业大学学报
%D 2012
%I 
%X The tobacco bacterial wilt disease caused by Ralstonia solanacearum is one of the important diseases of tobacco in China,and influences the field and quantity of tobacco leaves.Six hundred samples of root soil were collected from the serious disease areas in Yunnan and Guizhou,and screened SH7 strain showed strong antagonistic activity against R.solanacearum.The result showed that the inhibition zone width against R.solanacearum was 13.2 mm in the antibacterial test plate of laboratory.The control effect of the sterile fermentation broth of SH7 strain against R.solanacearum was 66.0% by pretreatment of tobacco root.The inhibition of crude protein extraction after precipitation and dialysis by 70%(NH4)2SO4 was strong with 15.7 mm of inhibition zone width and the inhibition determination of supernatant after removing precipitation by 70%(NH4)2SO4 was weak,which indicated that the antifungal substance was possibly protein.The result of phenotypic and general physiological and biochemical determination and 16s rDNA sequence analysis showed that SH7 strain belonged to one strain of B.subtilis.The production availability of B.subtilis SH7 strain was also discussed in this study.
%K tobacco bacterial wilt disease
%K Ralstonia solanacearum
%K Bacillus subtilis
%K biological control<br>烟草青枯病
%K 青枯雷尔氏菌
%K 枯草芽孢杆菌
%K 生物防治
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=74C612B26EF38BF0D4C997E3F9E167A1&aid=D812A4F49570FC505AB1989FD59C0B22&yid=99E9153A83D4CB11&vid=339D79302DF62549&iid=CA4FD0336C81A37A&sid=286FB2D22CF8D013&eid=11B4E5CC8CDD3201&journal_id=1000-5684&journal_name=吉林农业大学学报&referenced_num=0&reference_num=0