%0 Journal Article
%T Development of microsatellite markers for Lentinula edodes, using database search and ISSR-suppression PCR methods<br>数据库搜索及ISSR-抑制PCR法开发香菇微卫星标记
%A XIAO Yang
%A CHEN Long-Zhong
%A WU Qian
%A BIAN Yin-Bing
%A <br>肖扬
%A 陈隆钟
%A 吴茜
%A 边银丙
%J 菌物学报
%D 2009
%I 
%X Two methods,database search and ISSR-suppression PCR,were used to isolate microsatellite markers from Lentinula edodes.Twenty-one primer pairs were designed by database search,and eleven of them showed polymorphism.The number of alleles produced by these eleven markers was 3.3 per locus in average.Using method of ISSR-suppression PCR,eight primer pairs were developed and five of them had polymorphism.There were 3 alleles per locus in average produced by these five markers.The results showed that two methods...
%K Lentinula edodes
%K simple sequence repeat
%K database search
%K ISSR-suppression PCR<br>香菇
%K 简单系列重复
%K 数据库搜索
%K ISSR-抑制PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=BC5D5E417925E38F094185CE5ECD70FA&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=E5D85F291CED2DA6&eid=385E3C2062167B88&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=19