%0 Journal Article
%T A METHOD OF DETECTION FOR SUGAR AND ANTIGEN SITES ON HYPHAE SURFACES OF WHEAT STEM RUST BY PRE-EMBEDDING CYTOCHEMICAL TECHNIQUE<br>小麦秆锈菌菌丝体表糖基、抗原位点细胞化学测定方法的研究
%A KANG ZHEN-SHENG
%A LI ZHEN-QIPlant protection
%A <br>康振生
%A 李振岐
%A R．罗林格
%A J．庄约兰
%J 菌物学报
%D 1991
%I 
%X This paper described a method of detection for sugar and antigen sites on hyphae surfaces of wheat stem rust by pre-embedding cytochemical technique.After rust- infected wheat leave had been pre-fixed with 1% glutaraldehyde and 4% paraformallehyde,the sliced leaf samples were incubated with 2 lectin probes,or 2 antibodies and protein A-gold res- pectively,then following by post-fixation with 2% OsO_4,dehydration in a graded ethanol se- ries and embedding in Epon-Aratdite.It was observed that 2 kinds of sugar sides on the sur- faces of rust hyphae located between host cells had been accurately detected by 2 lectin probes, and 2 kinds of antigenic sites on the hyphae surfaces had been revealed by 2 antibodies and pro- tein A-gold.The result here indicates obviously that this method is suitable for detection of components on surfaces of rust hyphae located between host cells.
%K Colloidal gold
%K Cytochemistry
%K Hyphae surface
%K Sugar side
%K Antigen<br>胶体金
%K 细胞化学
%K 菌丝体表
%K 糖基
%K 抗原位点
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=7085F4C3ADDB58AFB60005224ADF63E2&yid=116CB34717B0B183&vid=F3090AE9B60B7ED1&iid=E158A972A605785F&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0