%0 Journal Article
%T Appressorial differentiation and its association with cAMP in the insect pathogenic fungus Metarhizium anisopliae<br>金龟子绿僵菌附着胞分化及其与环腺苷酸cAMP的关联性研究
%A DUAN Zhi-Bing
%A GAO Qiang
%A LU Ding-Ding
%A SHI Shao-Hua
%A BUTT Tariq M
%A WANG Cheng-Shu
%A <br>段志兵
%A 高强
%A 吕丁丁
%A 石少华
%A BUTT Tariq M.
%A 王成树
%J 菌物学报
%D 2009
%I 
%X Host recognition and appressorium differentiation are the pivotal steps for insect pathogenic fungi to initiate infection process. In this study, appressorial differentiation was studied by comparing a mutant, which has been identified with the loss of genetic materials, with the wild-type strain of the insect pathogenic fungus Metarhizium anisopliae. The observations showed that both the mutant and wild-type strain could not only produce appressoria on the tips of newly germinated spores, but also form multiple infection structures from the terminals of branching mycelia on insect cuticle. In contrast to the wild-type, the frequency of appressorial formation was significantly reduced and in addition, no clear mucilaginous sheath was produced by the mutant appressoria. The study shows that the cuticle degrading enzyme subtilisin is not involved in appressorial differentiation, or indispensable in cuticle digestion. A significantly low frequency of appressorial differentiation by the mutant was coincided with its lower intracellular cAMP level in comparison to the wild-type. Addition of exogenous cAMP could significantly increase the frequency of appressorial differentiation by the mutant, indicating that cAMP signaling pathway is potentially involved in regulation of appressorial differentiation in M. anisopliae.
%K Insect pathogenic fungus
%K cuticle penetration
%K mucilaginous sheath
%K subtilisin<br>昆虫病原真菌，体壁穿透，粘液层，类枯草杆菌蛋白酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=1B0917E1CA2F8461561735F5BF7AE354&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=94C357A881DFC066&sid=AB720B703F452703&eid=E5ED9059DE792E50&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=22