%0 Journal Article
%T Study on differential development gene of Pholiota adiposa bv DDRT-PCR<br>运用mRNA差异显示技术研究黄伞发育相关基因
%A JI Zhe
%A LI Yu-Xiang
%A ZHAO Ming-Wen
%A PAN Ying-Jie
%A <br>季哲
%A 李玉祥
%A 赵明文
%A 潘迎捷
%J 菌物学报
%D 2007
%I 
%X RNA of different development periods of pholiota adiposa including mycelium,anlage,bud and fruit body(stem and pileus of mushroom)was extracted and treated by RT-PCR.Four fragments(T_(11)G B0304,T_(11)G B0304-2,T_(11)G B0322 and T_(11)C B0310)were isolated,reamplified,purified,cloned and sequenced.After blasting in GenBank,T_(11)G B0322 cDNA fragment was identified to be similar to oxysterol-binding protein.The result of semiquantitive PCR showed that the expression of the T_(11)G B0322 cDNA fragment was different in different periods.This showed that gene fragment might be associated with differentiation of Ph.adiposa.
%K different cDNA fragment
%K oxysterol-binding protein
%K semiquantitive PCR<br>差异cDNA片段
%K 氧类固醇结合蛋白
%K 半定量PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=0D82FB21D3F89BF53715E5965A82D613&yid=A732AF04DDA03BB3&vid=96C778EE049EE47D&iid=0B39A22176CE99FB&sid=B78CD622C1934236&eid=FBA00558C57D9C11&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=35