%0 Journal Article
%T Two-dimensional electrophoresis and extraction methods of total protein of Ganoderma lucidum primordium<br>灵芝子实体原基双向电泳和总蛋白质提取方法的建立
%A LIU Xiao-Yun
%A SU Ming-Sheng
%A YANG Jun-Zhang
%A XIE Xiao-Mei
%A <br>刘晓云
%A 苏明声
%A 杨俊璋
%A 谢小梅
%J 菌物学报
%D 2009
%I 
%X The total protein extraction effect of Ganoderma lucidum primordium by Tris-saturated phenol law and trichloroacetic acid (TCA)/acetone precipitation methods were compared. After analyzing the two-dimensional electrophoresis (2-DE) protein atlas by the software of Image Master 2D Platinum 6.0, 565 and 273 protein spots were obtained separately by the method of Tris-saturated phenol and TCA/acetone precipitation; The 2-DE gels of TCA/acetone precipitation showed strong horizontal streaks in the atlas of alkalinity end and low molecular region, while the Tris-saturated phenol law was capable of generating more protein spots, obtaining better effect of isoelectric focusing and reducing salt. Tris-saturated phenol law can be considered as a feasible protein extraction method for Ganoderma lucidum primordium and the results of this study are of great significance for developing effective protein extraction methods in other medicinal mushrooms and will lay the foundation of further study on the discrepancy expression of Ganoderma lucidum treated by bi-directional solid fermentation.
%K Tris-saturated phenol law
%K TCA/acetone precipitation method
%K medicinal mushroom
%K bi-directional solid fermentation<br>Tris-饱和酚法，三氯乙酸/丙酮沉淀法，药用真菌，双向性固体发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=08D0673221AD7784064A755A38CAB32A&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=B31275AF3241DB2D&sid=4AB2A4AB67F2B16E&eid=8C8D39B86A1EED4F&journal_id=1672-6472&journal_name=菌物学报&referenced_num=1&reference_num=15