%0 Journal Article
%T PROTOPLAST FORMATION AND REGENERATION OF THE MOSQUITO FUNGAL PATHOGEN LAGENIDIUM GIGANTEUM (OOMYCETES: LAGENIDIALES)<br>灭蚊真菌——大链壶菌原生质体形成和再生的研究
%A He Shehui Su Xiaoqing Guiyang Medical College
%A Guiyang
%A <br>何社辉
%A 苏晓庆
%J 菌物学报
%D 1995
%I 
%X 采用1％纤维素酶与1％真菌脱壁酶混合液作脱壁酶，0.6mol／L山梨醇为渗透压稳定剂，从摇床培养的12一14小时菌龄的大链壶菌(Lagenidium giganteum)菌丝体获得原生质体。酶解3—5小时后，产量可达1.4—2.0×106／mL。并在双层培养基上初步实现了原生质体再生。
%K Lagenidium giganteum
%K protoplast
%K mosquito biocontrol<br>大链壶菌，原生质体，生物灭蚊
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=CF060D630AEF17F6BBF0146451C5D77A&yid=BBCD5003575B2B5F&vid=F3583C8E78166B9E&iid=38B194292C032A66&journal_id=1672-6472&journal_name=菌物学报&referenced_num=2&reference_num=0