%0 Journal Article
%T An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes
%A Mei Jing Piao
%A Yu Jae Hyun
%A Suk Ju Cho
%A Hee Kyoung Kang
%A Eun Sook Yoo
%A Young Sang Koh
%A Nam Ho Lee
%A Mi Hee Ko
%A Jin Won Hyun
%J Marine Drugs
%D 2012
%I MDPI AG
%R 10.3390/md10122826
%X The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisonia hamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO 4 + H 2O 2), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280¨C320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components.
%K Bonnemaisonia hamifera
%K human keratinocytes
%K photoprotection
%K reactive oxygen species
%K ultraviolet B
%U http://www.mdpi.com/1660-3397/10/12/2826