%0 Journal Article
%T Application of ISSR marker in the genetics analysis of monokaryons from Auricularia auricula<br>ISSR标记在黑木耳单核体遗传分析中的应用
%A SONG Xiao-Y
%A XIAO Yang
%A BIAN Yin-Bing
%A <br>宋小亚
%A 肖扬
%A 边银丙
%J 菌物学报
%D 2007
%I 
%X Two parent monokaryons (T1, T2) and thirty-three of F1 sporulated monokaryons have been developed from cultivated strain XinKe 5 of Auricularia auricula by protoplast monokaryonization and single-spore isolation technique. Seventy-three ISSR primers were screened and thirteen which could differentiate T1 and T2 effectively were selected to amplify strain XinKe 5 and F1 sporulated monokaryons. A total of seventy fragments were amplified, among them sixty-three (accounting for 90.0% of the total) were polymorphic. Genetic similarity coefficient matrix (GS) among thirty-six strains ranged from 0.2500 for the lowest similarity coefficient between parent monokaryotic strains T1 and T2 to 0.8382 for the greatest extent of similarity. The ISSR dendrogram based on the similarity coefficient matrix was constructed by using NTsys 2.10e software. Tested strains could be classified into 2 major groups: T1 and twenty-four of F1 sporulated monokaryons fell into same group, while T2 and the rest of F1 sporulated monokaryons fell into the other group. Monokaryons were notclassified strictly based on mating types. The results showed that chromosome crossing-over in each basidia of fruitbody was complex and polymorphic during meiosis, and the most of F1 sporulated monokaryons resembled parental monokaryon T1. The potential applications of ISSR marker were discussed in cross breeding of Auricularia auricula.
%K cultivated strain
%K genetic similarity coefficient
%K mating type factor<br>栽培菌株
%K 遗传相似系数
%K 交配型因子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=E726F06BC6A7E3270D5B7C224D9164C6&yid=A732AF04DDA03BB3&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=652E7E360EBE3082&eid=FF58680609C9D068&journal_id=1672-6472&journal_name=菌物学报&referenced_num=1&reference_num=31