%0 Journal Article
%T SCREENING OF CATABOLITE REPRESSION-RESISTANT MUTANTS OF CELLULASE PRODUC1NG PENICILLIUM SPP<br>青霉的纤维素酶抗降解物阻遏突变株的选育
%A QU Yin-bo
%A GAO Pei-ji
%A WANG Zu-nong Institute of Microbiology
%A Shandong University
%A Jinan
%A <br>曲音波
%A 高培基
%A 王祖农
%J 菌物学报
%D 1984
%I 
%X A screening method of double layer agar plate was improved by using holocellulose instead of acid-swollen cellulose as substrate. Using this method, a high cellulase producing fungus Penicillium decumbens 114-2 was isolated from the soil. The strain 114-2 was continually mutated with UV and NG. A series of catabolite- derepression mutants were selected on the holocellulose plate in the presence of excessive glucose. These mutants can hydrolyze holocellulose in the plate, but neither the parent strain 114-2 nor two of the high cellulase producing mutants (Trichoderma EA_3-867 and QM9414) have tais ability. Among the non-repressible cellulase producing mutants, two strains, JN15 and JU1, clearly produced a great amount of cellulase in the liquid holocel- lulose-glucose medium. In addition, when JU1 was grown on a medium containing holocellulose as the sole carbon source, the sasccharifying cellulase activities of JU1 on filter paper, CMC and cotton were as high as 33 mg glucose/ml. h, 234 mg glucose/ml.h and 86 mg glucose/ml.24h respectively. The cellulase production of P. decumbens strains, especially in JU1, is more sensi- tive to low pH. Thus, the addition of CaCO_3 is favorable.
%K Penicillium decumbens
%K cellulase
%K catabolite repression-resistant mutants<br>青霉
%K 纤维素酶
%K 抗降解物阻遏突变株
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=3CE988FD0686B3211F3AB5210049B507&yid=36250D1D6BDC99BD&vid=38B194292C032A66&iid=E158A972A605785F&journal_id=1672-6472&journal_name=菌物学报&referenced_num=17&reference_num=0