%0 Journal Article
%T INDUCED FORMATION, PURIFICATION AND PROPERTIES OF GLYCEROL-3-PHOSPHATE DEHYDROGENASE FROM SACCHAROMYCES CEREVISIAE<br>酿酒酵母3-磷酸甘油脱氢酶的诱导、提纯和性质
%A Cai Jingmin
%A <br>蔡敬民
%J 菌物学报
%D 1996
%I 
%X 在YEPD培养基中添加NaCl，可以诱导酿酒酵母(Saccharomyces cerevisiae)细胞内3-磷酸甘油脱氢酶的形成，当NaCl浓度达5％时，酶比活从0．05U／mg提高到0.5U／mg；若再限制培养基中葡萄糖浓度在100mg／L以下，酶比活可达到0．89U／mg。酶比活与培养基中的NaCl浓度的函数关系式为：Sa-0.129C3-0.038C2+0.034C+0.063(O≤C≤5％)。粗酶液经Sephadex G一25凝胶过滤，Blue Sepharose亲和层析以及Mono Q离子交换等步骤，提纯123．6倍，得纯酶液。经SDS一凝胶电泳测得分子量为45 000±2 000。酶的最适温度为51℃，最适pH值为6.8。保温30分钟的半失活温度(t1/2)为41℃。NADH和DHAP的Km(mmol／L)值分别为：0.0177和0.134。
%K Saccharomyces cerevisiae
%K Glycerol - 3 - phosphate dehydrogenase
%K Enzyme induced formation
%K Purification and properties<br>酿酒酵母，3一磷酸甘油脱氢酶，酶的诱导，提纯和性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=4286E8F374586C1CCCC4A00F6442EF3F&yid=8A15F8B0AA0E5323&vid=23CCDDCD68FFCC2F&iid=0B39A22176CE99FB&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0