%0 Journal Article
%T Cloning of a gene encoding lipase from Thermomyces lanuginosus and effective expression of the gene in Pichia pastoris<br>疏棉状嗜热丝孢菌脂肪酶基因的克隆及其在毕赤酵母中的高效表达
%A ZHENG Yan
%A ZHOU Bo
%A SONG Ning-Ning
%A LI Duo-Chuan
%A <br>郑艳
%A 周波
%A 宋宁宁
%A 李多川
%J 菌物学报
%D 2009
%I 
%X Thermomyces lanuginosus produces an extracellular lipase that is important for industrial applications. Based on published DNA sequence, we cloned the full length genomic DNA and cDNA of the lipase using PCR and RT-PCR methods. DNA sequencing revealed that the genomic DNA of the T. lanuginosus lipase gene has an open reading frame of 876bp encoding a protein lipase precursor of 292 amino acid residues, and three introns. And the cDNA of lipase gene we cloned is identical to the registered lipase gene of The...
%K intron
%K mature protein
%K induction
%K secretive expression
%K recombinant enzyme
%K optimal temperature
%K optimal pH
%K thermostability<br>内含子
%K 成熟蛋白
%K 诱导
%K 分泌表达
%K 重组酶
%K 最适反应温度
%K 最适反应pH
%K 热稳定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=FD95E1256D11BE139939730355AC78B6&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=DC330B09A33F1455&eid=6826CBE9C80ACB20&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0