%0 Journal Article
%T Applicability of PCR-DGGE and 16S rDNA Sequencing for Microbiological Analysis of Otitis Media with Effusion
%A Priit Kasen£¿mm
%A Jelena £¿t£¿epetova
%J International Journal of Otolaryngology and Head & Neck Surgery
%P 71-76
%@ 2168-5460
%D 2012
%I Scientific Research Publishing
%R 10.4236/ijohns.2012.13015
%X <b>Background:</b> The aim of the study was to analyze the performance of PCR-DGGE based assay and its applicability as a tool for the identification of bacteria in the middle ear of children with otitis media with effusion (OME). <b>Methods:</b> The middle ear effusions from 20 children with OME were analyzed both by bacterial culture and by 16S rDNA-gene-targeted PCR assay, DGGE fingerprinting and sequencing analysis. <b>Results:</b> In bacterial culture assay, only three middle ear effusions (15%) showed bacterial growth. None of the samples were positive for anaerobic culture. The PCR assay with 16S rDNA-gene-targeted universal primers was positive in 10 (50%) cases. The subsequent DGGE fingerprinting and 16S rDNA sequencing analysis revealed that the most commonly encountered bacteria in the middle ear effusions of children with OME are <i>Haemophilus influenzae, Alloiococcus otitidis</i> and <i>Bacteroides</i> spp. <b>Conclusions:</b> The present study demonstrated the applicability of PCR-DGGE based assay and 16S rDNA sequencing for analyzing of bacterial diversity in the middle ear effusion of children OME. The results of our study may contribute to a better understanding of the etiology of OME.
%K Otitis Media with Effusion
%K 16S rDNA Targeted PCR
%K DGGE Fingerprinting
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=24785