%0 Journal Article
%T Localization of Active Caspase-3 and Caspase-8 in Nasal Polyps and Nasal Hyperplasia in Consideration of Mast Cell Function: A Semiquantitatively Analysis
%A Nadine Franzke
%A Sibylle Koehler
%A Peter Middel
%A Claudia Fuoco
%A Francesco Cecconi
%A Fabio Quondamatteo
%A Rainer Laskawi
%A Saskia Rohrbach
%J International Journal of Otolaryngology and Head & Neck Surgery
%P 63-70
%@ 2168-5460
%D 2012
%I Scientific Research Publishing
%R 10.4236/ijohns.2012.13014
%X <b>Introduction:</b> The pathogenesis of nasal polyposis and nasal hyperplasia is still unknown. The localization of caspases in nasal polyps and nasal hyperplasia of patients with and without allergic rhinitis was studied. <b>Methods:</b> Sections of human nasal polyps (n = 5) and hyperplastic nasal turbinates (5 with, 5 without allergy) were stained for active caspase-3 and caspase-8. Double immunofluorescence was used to evaluate colocalization of the caspases with Ki-M1P and tryptase. TUNEL was performed. <b>Results:</b> Active caspase-3 and caspase-8 were seen in nearly all nasal polyps and hyperplastic nasal turbinates. Active caspase-3 was predominantly localized in stromal cells, identified as mast cells. Caspase-8 was localized in mast cells with the pattern similar to active caspase-3 and additionally found in epithelial cells at the nasal and polyp surface and in epithelial cells of glands. <b>Conclusion:</b> Our results suggest that mast cell apoptosis may be involved in the pathological mechanisms which characterize and sustain chronic inflammatory disorders of the nasal mucosa with and without allergy.
%K Nasal Polyposis
%K Caspase-3
%K Caspase-8
%K Allergic Rhinitis
%K Non-Allergic Rhinitis
%K Mast Cells
%K Nasal Pathology
%K Chronic Rhinosinusitis
%K Apoptosis
%K Nasal Hyperplasia
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=24780