%0 Journal Article
%T Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples
%A Qi Zhang
%A Wen Zhang
%A Xiuping Wang
%A Peiwu Li
%J Molecules
%D 2010
%I MDPI AG
%R 10.3390/molecules15010164
%X Five generic haptens of pyrethoid insecticides, which were classified as three types, were designed and synthesized: the first (hapten 1) is for type I pyrethroids without a cyano group, the second (hapten 2 and XQ) for type II pyrethroids with a cyano group, and the third (hapten 4 and 5) for both types of pyrethroids with loss of the ester group. The hapten structures were confirmed by MS and 1H-NMR. Hapten 1 and 2 were conjugated with BSA respectively and haptens 1-5 were conjugated with OVA. Four polyclonal antisera were raised against BSA conjugates including a mixture conjugate, and twenty antibody/coating conjugate combinations were selected for studies of assay sensitivity and specificity for pyrethroids. The study revealed the best combination, which showed equal high sensitivities (I50 is around 0.02 ¦Ìg mL-1) to both types of pyrethroids. The immunity results suggest that, with a mixture conjugates, a polyclonal antibody against a group of insecticides can be prepared for multi-residue assays.
%K hapten
%K class-specific antibody
%K sensitivity
%K immunoassay
%K pyrethoid insecticides
%U http://www.mdpi.com/1420-3049/15/1/164