%0 Journal Article
%T Effects of Anonaine on Dopamine Biosynthesis and L-DOPA-Induced Cytotoxicity in PC12 Cells
%A Jae Joon Lee
%A Chun Mei Jin
%A Young Kyoon Kim
%A Shi Yong Ryu
%A Sung Cil Lim
%A Myung Koo Lee
%J Molecules
%D 2008
%I MDPI AG
%R 10.3390/molecules13020475
%X The effects of anonaine, an aporphine isoquinoline alkaloid, on dopaminebiosynthesis and L-DOPA-induced cytotoxicity in PC12 cells were investigated. Anonaineat concentration ranges of 0.01-0.2 ¦ÌM showed a significant inhibition of dopaminecontent at 24 h, with an IC50 value of 0.05 ¦ÌM. Anonaine at 0.05 ¦ÌM inhibited tyrosinehydroxylase (TH) and aromatic L-amino acid decarboxylase (AADC) activities to 38.4-40.2% and 78.4-90.2% of control levels at 12-24 h and 3-6 h, respectively. TH activity wasmore influenced than AADC activity. Anonaine also decreased intracellular cyclic AMPlevels, but not intracellular Ca2+ concentrations. In addition, anonaine (0.05 ¦ÌM) reducedL-DOPA (50 ¦ÌM and 100 ¦ÌM)-induced increases in dopamine content at 24 h. However,anonaine (0.05 ¦ÌM) did not enhance L-DOPA (50 ¦ÌM and 100 ¦ÌM)-induced cell death 476after 24 h. These results suggest that anonaine inhibits dopamine biosynthesis by mainlyreducing TH activity without aggravating L-DOPA-induced cytotoxicity in PC12 cells.
%K Anonaine
%K Dopamine biosynthesis
%K L-DOPA-induced cytotoxicity
%K PC12 cells.
%U http://www.mdpi.com/1420-3049/13/2/475