%0 Journal Article
%T Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches<br>
%A Decai Jin
%A Ping Wang
%A Zhihui Bai
%A Xinxin Wang
%A Hong Peng
%A Rong Qi
%A Zhisheng Yu
%A Guoqiang Zhuang
%A <br>
%J 环境科学学报(英文版)
%D 2011
%I 
%X The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking. A total of 28 species were obtained from 63 isolates collected from six culture media. The most cultivable species belonged to γ-Proteobacteria including Klebsiella sp., Pseudomonas sp., Aeromonas sp. and Acinetobacter sp. Further analysis of these strains by repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets. While the culture-independent assessment revealed that β-Proteobacteria was the dominant group in the bulking sample. Sequence analysis revealed that the highest proportion (14.7%) of operational taxonomic units was 98% similar to Candidatus Accumulibacter phosphatis, which is used to remove phosphorous from wastewater. Our results indicated that combining différent approaches can produce complementary information, thus generate a more accurate view of microbial community in bulking sludge.
%K bulking sludge
%K cultivable bacteria
%K rep-PCR
%K 16S rRNA gene clone library<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=1F8EB868F38CE0725815032E14D0AE66&yid=9377ED8094509821&vid=EA389574707BDED3&iid=708DD6B15D2464E8&sid=3907BD093A0059AA&eid=9D3954EE0D6BC193&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=32