%0 Journal Article
%T Mesoporous silicas synthesis and application for lignin peroxidase immobilization by covalent binding method
%A Zunfang Hu
%A Longqian Xu
%A Xianghua Wen
%A <br>Zunfang Hu
%A Longqian Xu
%A Xianghua Wen
%J 环境科学学报(英文版)
%D 2013
%I 
%X Immobilization of enzymes on mesoporous silicas (MS) allows for good reusability. MS with two-dimensional hexagonal pores in diameter up to 14.13 nm were synthesized using Pluronic P123 as template and 1,3,5-triisopropylbenzene as a swelling agent in acetate buffer. The surface of MS was modified by the silanization reagents 3-aminopropyltriethoxysilane. Lignin peroxidase (LiP) was successfully immobilized on the modified MS through covalent binding method by four agents: glutaraldehyde, 1,4-phenylene diisothiocyanate, cyanotic chloride and water-soluble carbodiimide. Results showed that cyanotic chloride provided the best performance for LiP immobilization. The loaded protein concentration was 12.15 mg/g and the immobilized LiP activity was 812.9 U/L. Immobilized LiP had better pH stability. Acid Orange II was used to examine the reusability of immobilized LiP, showing more than 50% of the dye was decolorized at the fifth cycle.
%K mesoporous silica
%K lignin peroxidase
%K covalent binding
%K stability<br>mesoporous
%K silica
%K lignin
%K peroxidase
%K covalent
%K binding
%K stability
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=2DB3B6D3544EDEBD826DE4EE9742E8A1&yid=FF7AA908D58E97FA&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=7EBE588F611589FC&eid=3E0812ED84A7B31D&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=30