%0 Journal Article %T Carbendazim induces a temporary change in soil bacterial community structure
%A WANG Xiuguo %A SONG Min %A GAO Chunming %A DONG Bin %A ZHANG Qun %A FANG Hua %A YU Yunlong %A
%J 环境科学学报(英文版) %D 2009 %I %X The effect of carbendazim applications on the diversity and structure of a soil bacterial community was studied under field conditions using temperature gradient gel electrophoresis (TGGE) and partial sequence analysis of PCR-amplified 16S rRNA gene. After four successive introductions of carbendazim at a level of 0.94 kg active ingredient (a.i.)/ha, the genetic diversity (expressed as Shannon index, H0) decreased from 1.43 in the control to 1.29 in treated soil. This harmful e ect seems to increase with the concentration of carbendazim. The value of H0 in the soil treated with carbendazim at 4.70 kg a.i./ha was reduced to 1.05 (P 6 0.05). The structure of soil bacterial community was also a ected after four repeated applications of carbendazim at levels of 0.94, 1.88 and 4.70 kg a.i./ha, as seen in the relative intensities of the individual band. However, the bacterial community in carbendazim-treated soil recovered to that in the control 360 d after the first treatment. The results indicated that repeated applications of carbendazim could reduce soil microbial diversity and alter the bacterial community structure temporarily. %K carbendazim %K repeated applications %K bacterial diversity %K temperature gradient gelelectrophoresis %K phylogenetic analysis
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=D49E71F58685DAA0FED991FDB995A0FB&yid=DE12191FBD62783C&vid=659D3B06EBF534A7&iid=59906B3B2830C2C5&sid=0C0A5470C59ABA43&eid=E652F68A3FDC0E89&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=1&reference_num=27