%0 Journal Article
%T Determination of hemoglobin adduct of a musk xylene metabolite in trout as biomarker of exposure by gas chromatography mass spectrometry
%A MOTTALEB Mohammad Abdul
%A KARIM Mohammad Rezaul
%A <br>MOTTALEB Mohammad Abdul
%A KARIM Mohammad Rezaul
%J 环境科学学报(英文版)
%D 2005
%I 
%X Musk xylene(MX) is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-amino-MX(4-AMX) as cysteine adducts in trout hemoglobin(Hb) was made by gas chromatography-ion trap-mass spectrometry(GC/MS). The Hb samples were collected from trout after 24 h exposure to MX at 10 microg/g, and or menhaden oil(control). The formation of cysteine-Hb adduct was observed from nitroso derivative of MX, released by alkaline hydrolysis. The released 4-AMX metabolite was extracted in n-hexane. The extract was then reduced by evaporation, and analyzed by GC/MS. When similar agreement of mass spectral features and retention time of 4-AMX were obtained in both standard and sample solutions, the presence of 4-AMX metabolite in the Hb was confirmed. The concentration of 4-AMX was found to be 3.1 x 10(-6)--6.9 x 10(-6) mg/g in the Hb solution. Quantitation was made based on an internal standard, a calibration plot, and response factor. In the non-hydrolyzed and laboratory blank extracts, the 4-AMX metabolite was not detected. Additionally, coeluting and interfering ions were observed in the biological samples.
%K biomarkers
%K musk xylene metabolite
%K Hb adducts
%K fish exposure<br>血色素
%K 二甲苯
%K 代谢物
%K 光谱测定法
%K 淤泥
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=74123665A3916B23C576DC16E348C705&yid=2DD7160C83D0ACED&vid=BCA2697F357F2001&iid=B31275AF3241DB2D&sid=A73A882009D0AEFE&eid=E1034A3BCFB43055&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=27