%0 Journal Article
%T Assessing the anti-estrogenic activity of sodium pentachlorophenol in primary cultures of juvenile goldfish (Carassius auratus) hepatocytes using vitellogenin as a biomarker
%A ZHAO Bing
%A LIU Zheng-tao
%A XU Zhang-fa
%A <br>ZHAO Bing
%A LIU Zheng-tao
%A XU Zhang-fa
%J 环境科学学报(英文版)
%D 2006
%I 
%X Both pentachlorophenol and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) had been studied widely because of their probable anti-estrogenic activity. Sodium pentachlorophenol (PCP-Na), as a industrial product used in many fields, usually contains a trace of TCDD. The aim of this study was to assess the anti-estrogenic effect of PCP-Na in juvenile goldfish (Carassius auratus) hepatocyte cultures using vitellogenin (VTG) as the biomarker. The ID50 of PCP-Na was investigated and then a series of concentrations (0.001-0.5 microg/ml) of PCP-Na were evaluated to estimate the anti-estrogenic activity. Results showed that PCP-Na was cytotoxic for hepatocytes even at very low concentration < 1.21 microg/ml, and it could not induce VTG at any concentrations tested. Since it failed to stimulate VTG production, the possibility of its anti-estrogenic effect was tested, and a well-known anti-estrogenic compound-tamoxifen was used as positive control. PCP-Na caused a reduction in VTG synthesis in juvenile goldfish (Carassius auratus) hepatocytes at concentrations > 0.1 microg/ml when co-exposure with 1 microg/ml 17beta-estradiol (E2), making its anti-estrogenic activity approximately as potent as tamoxifen. Our results indicate that PCP-Na can act as negative modulators of estrogenic function in juvenile goldfish (Carassius auratus) hepatocytes.
%K PCP-Na
%K vitellogenin
%K juvenile goldfish
%K hepatocytes
%K anti-estrogenic activity<br>卵黄蛋白原
%K 金鱼
%K 肝细胞
%K 雌激素
%K 环境动物学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=35C89418A04A0E0D5CEEBF66ADB818D7&yid=37904DC365DD7266&vid=13553B2D12F347E8&iid=38B194292C032A66&sid=89389F643CA3F778&eid=500A64A3035F6B9C&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=30