%0 Journal Article
%T Biodegradation of azo dyes by genetically engineered azoreductase
%A WANG Jing
%A YAN Bin
%A ZHOU Ji-ti
%A BAO Yong-ming
%A LU Hong
%A YUAN Xiao-dong
%A <br>WANG Jing
%A YAN Bin
%A ZHOU Ji-ti
%A BAO Yong-ming
%A LU Hong
%A YUAN Xiao-dong
%J 环境科学学报(英文版)
%D 2005
%I 
%X A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.
%K genetically engineered microorganisms
%K azoreductase
%K biodegradation
%K azo dyes<br>基因工程
%K 微有机物
%K 生物降解
%K PCR放大
%K 含氮染色体
%K 废物处理
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=C215A3124845D68DBF96706242D34523&yid=2DD7160C83D0ACED&vid=BCA2697F357F2001&iid=E158A972A605785F&sid=6313C162FF75889A&eid=64808317C39DF331&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=15