%0 Journal Article
%T Quantification of anaerobic ammonium-oxidizing bacteria in enrichment cultures by quantitative competitive PCR<br>
%A HAO Chun
%A WANG Huan
%A LIU Qinhua
%A LI Xudong
%A <br>
%J 环境科学学报(英文版)
%D 2009
%I 
%X The anaerobic ammonium-oxidizing (ANAMMOX) bacteria were enriched from a sequencing batch biofilm reactor (SBBR) biofilm.We successfully developed a quantitative competitive polymerase chain reaction (QC-PCR) system to detect and quantify ANAMMOX bacteria in environmental samples.For QC-PCR system,PCR primer sets targeting 16S ribosomal RNA genes of ANAMMOX bacteria were designed and used.The quantification range of this system was 4 orders of magnitude,from 10~3 to 10~6 copies per PCR,corresponding to the detection limit of 300 target copies per mL.A 312-bp internal standard (IS) was constructed,which showed very similar amplification efficiency with the target amxC fragment (349 bp) over 4 orders of magnitude (10~3-10~6).The linear regressions were obtained with a R~2 of 0.9824 for 10~3 copies,R~2 of 0.9882 for 10~4 copies,0.9857 for 10~5 copies and 0.9899 for 10~6 copies.Using this method,we quantified ANAMMOX bacteria in a shortcut nitrification/denitrification-anammox system which is set for piggery wastewater treatment.
%K anaerobic ammonium oxidation
%K 16S rRNA approach
%K quantitative competitive PCR<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=79A06EBA2D88194DFF9A37A25251E070&yid=DE12191FBD62783C&vid=659D3B06EBF534A7&iid=708DD6B15D2464E8&sid=CDD86F65AA3A5D34&eid=E0EA1E85C42D382D&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=22