%0 Journal Article
%T Ultraviolet irradiation induced oxidative stress and response of antioxidant system in an intertidal macroalgae Corallina o cinalis L.
%A Lixia Li
%A Jiqiang Zhao
%A Xuexi Tang
%A
%J 环境科学学报(英文版)
%D 2010
%I
%X The response of the antioxidant defense system of an intertidal macroalgae Corallina o cinalis L. to di erent dosages of UV-B irradiation was investigated. Results showed that superoxide dimutase (SOD) and peroxidase (POX) increased and then maintained at a relatively stable level when subjected to UV-B irradiation. Catalase (CAT) activity under medium dosage of UV-B irradiation (Muv) and high dosage of UV-B irradiation (Huv) treatments were significantly decreased. Ascorbate peroxidase (APX) activity first remained unaltered and then increased in Huv treatment. In addition, the assay on isozymes was carried out using non-denaturing polyacrylamide gel electrophoresis (PAGE). The activities of some SOD isoforms were altered by UV-B. Two new bands (POX V and POX VII) appeared upon exposure to all three UV-B dosages. CAT III activity was increased by low dosage of UV-B irradiation (Luv), whereas CAT III and CAT IV disappeared when the alga was exposed to Muv and Huv. Two bands of APX (APX VI and APX VII) were increased and a new band (APX X) was observed under Huv exposure. H2O2 and thiobarbituric acid reacting substance (TBARS) increased under Muv and Huv treatments. Overall, UV-B protection mechanisms are partly inducible and to a certain extent su cient to prevent the accumulation of damage in C. o cinalis.
%K UV-B irradiation
%K Corallina o cinalis L
%K superoxide dimutase
%K catalase
%K ascorbate peroxidase
%K peroxidase
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=4F4304D8415925E698EFDC322E1B2D04&yid=140ECF96957D60B2&vid=BC12EA701C895178&iid=94C357A881DFC066&sid=27350781F1397F32&eid=D7F3FD6D87AEF622&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=0&reference_num=51