%0 Journal Article
%T Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity<br>Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa （Reeve） and its utility as a test species for sediment toxicity
%A Taowu M
%A Shuangjiao Gong
%A Ke Zhou
%A Cheng Zhu
%A Kaidong Deng
%A Qinghua Luo
%A Zijian Wang
%A <br>Taowu M
%A Shuangjiao Gong
%A Ke Zhou
%A Cheng Zhu
%A Kaidong Deng
%A Qinghua Luo
%A Zijian Wang
%J 环境科学学报(英文版)
%D 2010
%I 
%X This study aimed to develop original laboratory culture and sediment toxicity testing protocols for the freshwater gastropod Bellamya aeruginosa (Reeve), a new potential species for sediment toxicity testing. B. aeruginosa was successfully cultured with an e ective culture system under proposed laboratory conditions. Optimal ad libitum feeding levels for larvae, juveniles, and adults were 2.0, 6.0, and 16.0 mg fish food/(snail day), respectively. Mean survival rates of juveniles were higher than 90%. The snails could be sexed at 9 weeks of age, and their generation time is approximately 4 months. Reproduction continued all year around; the mean fecundity was 0.55 newborn/(female day). The utility of this species for bioassays was evaluated in both 10-day and 28-day case studies with artificial sediments. The 10-day LC50 of Cu for larvae was 480 g/g dry weight (dw), and the lowest observed e ects concentration of Cu for survival and growth of larvae was 195 g/g dw. Survival and growth are reliable indicators of acute toxicity. Larvae accumulated more Cu than adults. B. aeruginosa exhibited a higher sensitivity to Cu exposure than standard test species (Hyalella azteca and Chironomus tentans). The 28-day test of sediment toxicity with adults showed that fecundity was a robust endpoint indicator of reproductive toxicity, and the biochemical endpoints of superoxide dismutase, catalase, and glutathione could be used as sensitive biomarkers for Cu-induced oxidative damage. B. aeruginosa can be therefore recommended as a candidate for the standardization of the freshwater sediment toxicity test protocol.
%K gastropod
%K cultures
%K life history
%K lowest observed effect concentration
%K bioaccumulation
%K oxidative stress<br>铜锈环棱螺
%K 实验室培养
%K 水沉积物
%K 毒性测试
%K 物种
%K 超氧化物歧化酶
%K 未成年人
%K 幼虫生长
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=9C47DA07CE5696F417207778EB46B66B&yid=140ECF96957D60B2&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=8ED630AD8C61FAE8&eid=C2F76551C0111538&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=1&reference_num=34