%0 Journal Article
%T Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems
%A Xiaohui Wang
%A Xianghua Wen
%A Craig Criddle
%A George Wells
%A Jie Zhang
%A Yin Zhao
%A <br>Xiaohui Wang
%A Xianghua Wen
%A Craig Criddle
%A George Wells
%A Jie Zhang
%A Yin Zhao
%J 环境科学学报(英文版)
%D 2010
%I 
%X We investigated the communities of ammonia-oxidizing bacteria (AOB) in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction (PCR) followed by terminal restriction fragment length polymorphism (T-RFLP), cloning, and sequencing of the -subunit of the ammonia monooxygenase gene (amoA). The T-RFLP fingerprint analyses showed that di erent wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable di erence among the AOB TRFLP profiles from di erent parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant (WWTP) contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent a ected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little a ected by the treatment process in this study. Phylogenetic analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.
%K activated sludge
%K ammonia-oxidizing bacteria
%K T-RFLP
%K amoA gene
%K wastewater treatment plant<br>污水处理系统
%K 活性污泥
%K 氨氧化
%K 限制性片段长度多态性
%K 社区
%K 细菌
%K RFLP技术
%K 废水处理系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=A7CA601309F5FED03C078BCE383971DC&jid=6CB1530875F53489BF1E81BD87B7F5E6&aid=AA4EB84954AE8D467B696482053E4E94&yid=140ECF96957D60B2&vid=BC12EA701C895178&iid=E158A972A605785F&sid=821800203AD09E7B&eid=20ED669EB429E15C&journal_id=1001-0742&journal_name=Journalofenvironmentalsciences(China)&referenced_num=6&reference_num=37